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Histo 101: Stains, Slides, and Scopes

Community · Mixed · 20–30 min

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This board helps players review Histology through a Jeopardy-style game format on Kleveroo. It includes categories such as Tissue Prep & Sections, H&E and Dye Affinities, Special Stains & Targets, Immunostains & Microscopes, Slide Reading & Interpretation. It is useful for classroom review, team trivia, family game night, test prep, and group play.

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A peek at what's on the board — 5 categories and one sample clue from each. Answers stay hidden until you open them, so the game still works.

  • Tissue Prep & Sections

    This fixative is usually used to preserve tissue by crosslinking proteins.

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    formalin

    +4 more clues in this category

  • H&E and Dye Affinities

    This basic dye stains negatively charged structures like nuclei blue-purple.

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    hematoxylin

    +4 more clues in this category

  • Special Stains & Targets

    This stain colors carbohydrates and can show glycogen in hepatocytes.

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    PAS

    +4 more clues in this category

  • Immunostains & Microscopes

    This method uses a second antibody with a fluorophore to detect a specific protein.

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    immunofluorescence

    +4 more clues in this category

  • Slide Reading & Interpretation

    When reading slides, always compare what looks similar and what looks this.

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    different

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Board content

Tissue Prep & Sections

$100 — This fixative is usually used to preserve tissue by crosslinking proteins.
formalin
$200 — Fixation stops these two processes that would otherwise damage tissue.
metabolism and degradation
$300 — Without special steps, these molecules are washed out during processing and not stained with H&E.
lipids
$400 — Cut this way, thinner versions let you visualize more details by microscopy.
sections
$500 — Rapid assessment in surgery uses samples that are sectioned in this state.
frozen

H&E and Dye Affinities

$100 — This basic dye stains negatively charged structures like nuclei blue-purple.
hematoxylin
$200 — This acidic dye stains positively charged proteins in cytoplasm and muscle.
eosin
$300 — Another term for acidophilia seen with eosin-stained structures.
eosinophilia
$400 — Organelles and proteins stained with basic dyes show this property.
basophilia
$500 — Within the nucleus, this structure appears blue/purple with hematoxylin.
nucleolus

Special Stains & Targets

$100 — This stain colors carbohydrates and can show glycogen in hepatocytes.
PAS
$200 — This blood smear stain is used for peripheral blood or bone marrow.
Wright’s stain
$300 — This trichrome method typically shows muscle red and collagen green or blue.
Masson trichrome
$400 — This blue stain highlights iron deposits.
Prussian blue
$500 — These fibers of type III collagen are revealed by silver stain.
reticular fibers

Immunostains & Microscopes

$100 — This method uses a second antibody with a fluorophore to detect a specific protein.
immunofluorescence
$200 — This related method uses a chromogen to produce a color product.
immunohistochemistry
$300 — In immunostaining, you add this specific molecule that binds your target.
antibody
$400 — These two electron microscopy types complement light microscopy.
SEM and TEM
$500 — In H&E vs immunostain, a round nucleus in CT or epithelium points to this cell.
lymphocyte

Slide Reading & Interpretation

$100 — When reading slides, always compare what looks similar and what looks this.
different
$200 — A key question while scanning cells is where this structure is located.
nucleus
$300 — In nervous tissue, these smaller supporting cells are contrasted with larger neurons.
glial cells
$400 — On a Wright-Giemsa smear, these cytoplasmic inclusions correspond to lysosomes.
granules
$500 — Interpretation requires remembering you see this many-dimensional structure in two dimensions.
three-dimensional

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