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Histo 101: Stains, Sections, and Seeing

Community · Mixed · 20–30 min

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This board helps players review Histology Basics through a Jeopardy-style game format on Kleveroo. It includes categories such as Fixation & Frozen Sections, H&E and Dye Basics, Special Stains & Targets, Immuno Methods: IF vs IHC, Microscopy & Slide Interpretation. It is useful for classroom review, team trivia, family game night, test prep, and group play.

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A peek at what's on the board — 5 categories and one sample clue from each. Answers stay hidden until you open them, so the game still works.

  • Fixation & Frozen Sections

    This common fixative crosslinks proteins to stop tissue metabolism and degradation.

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    Formalin

    +4 more clues in this category

  • H&E and Dye Basics

    These two dyes are the workhorse stains of histology and pathology.

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    Hematoxylin and eosin

    +4 more clues in this category

  • Special Stains & Targets

    This stain highlights carbohydrates such as glycogen in hepatocytes.

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    PAS

    +4 more clues in this category

  • Immuno Methods: IF vs IHC

    These techniques use antibodies against specific proteins to label tissue sections.

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    Immunofluorescence and immunohistochemistry

    +4 more clues in this category

  • Microscopy & Slide Interpretation

    A basic slide-reading prompt asks where this key structure is located and what shape it has.

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    Nucleus

    +4 more clues in this category

Board content

Fixation & Frozen Sections

$100 — This common fixative crosslinks proteins to stop tissue metabolism and degradation.
Formalin
$200 — In routine fixation, these molecules are washed out and not stained without special steps.
Lipids
$300 — Cutting thinner sections after embedding improves this aspect of microscopic visualization.
Detail
$400 — These sections are frozen, cut, and stained for rapid assessment in surgery.
Frozen sections
$500 — This simple blue dye is used to rapidly stain frozen sections during surgery.
Methylene blue

H&E and Dye Basics

$100 — These two dyes are the workhorse stains of histology and pathology.
Hematoxylin and eosin
$200 — This basic, positively charged dye stains negatively charged structures like nuclei blue‑purple.
Hematoxylin
$300 — This acidic, negatively charged dye stains positively charged proteins in cytoplasm and muscle.
Eosin
$400 — This term describes structures that stain with basic dyes like hematoxylin.
Basophilia
$500 — Also called eosinophilia, this term describes affinity for acidic dyes.
Acidophilia

Special Stains & Targets

$100 — This stain highlights carbohydrates such as glycogen in hepatocytes.
PAS
$200 — In trichrome staining, this connective tissue component appears green or blue.
Collagen
$300 — This classic blue stain is used to demonstrate iron deposits.
Prussian blue
$400 — This metal-based stain highlights reticular fibers composed of type III collagen.
Silver stain
$500 — In neural tissue, this dye stains the myelin sheath blue.
Luxol blue

Immuno Methods: IF vs IHC

$100 — These techniques use antibodies against specific proteins to label tissue sections.
Immunofluorescence and immunohistochemistry
$200 — In immunofluorescence, the second antibody carries this light‑emitting tag.
Fluorophore
$300 — In immunohistochemistry, the second antibody produces color via this.
Chromogen
$400 — This type of stain uses antibodies to reveal a protein unique to your target cells.
Immunohistochemical stain
$500 — In the HeLa example, an antibody to this nuclear protein labels chromatin.
Histone H3

Microscopy & Slide Interpretation

$100 — A basic slide-reading prompt asks where this key structure is located and what shape it has.
Nucleus
$200 — When interpreting slides, remember you’re viewing this reduced to two dimensions.
Three-dimensional structure
$300 — In neurons, this dark purple spot within the nucleus is a key identifying feature.
Nucleolus
$400 — In Wright‑Giemsa smears, cytoplasmic granules correspond to these organelles.
Lysosomes
$500 — These three abbreviations label the microscopy modes compared in the lesson.
LM, TEM, SEM

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